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DNA-Technology
Equipment manufactured by "DNA-Technology" corporation

Real-Time PCR Cycler "DT-322"

Features of detecting amplifier DT-322

  • Possibility of a quantitative analysis of nucleic acids
  • Lack of an electrophoresis stage
  • Decrease of a chance of contamination
  • Essential acceleration of an analysis time

Real-Time PCR Cycler "DT-322" is intended for a carrying out of polimerase chain reaction (PCR) with a detection of an accumulation of PCR products during an amplification (Real -Time PCR). DT-322 allows to carry out the qualitative and quantitative PCR analysis excepting a stage of the electrophoresis of PCR products in an agarose gel. The device is designed for simultaneous analysis of up to 32 samples. A thermocycler and an optical system of a fluorescence detection are united in this device.

All data of the last amplification are recording into a private memory of the PCR Cycler and can be read out even after a current interruption occurred during an amplification. After a restoring of a power supply the amplification program will be continued, and the moment of interruption, its duration and a block temperature in the moment of interruption will be recorded. A turning off or a hanging of external computer does not result in PCR termination since the process control is managed by the private memory of the device that does not depend on a computer.

 
Technical parameters
technical certificate (PDF) | (DOC)

Thermoblock format

32 0,2 ml tubes (8 õ 4)

Tube type

0,2 ml (single or in the strips in 8)

Temperature range of a thermoblock

4…99 °Ñ

Discontinuity of a temperature adjustment

0.1 °Ñ

Unevenness of a thermoblock temperature, no more than

0.3 °Ñ

Heating rate of a thermoblock within a temperature range of 4…99 °Ñ

3 °Ñ /sec

Cooling rate of a thermoblock within a temperature range of 55…99 °Ñ2.5 °Ñ /sec
Absolute accuracy of a temperature maintenance, no more than0.3 °Ñ
Dynamic errors of a temperature regulation, no more than0.3 °Ñ
Executing unit of a thermoblockPeltier element
Thermoblock cover heatingyes
Excitation sourceLED
DetectorCCD-array
Number of channels to measure the fluorescence2
Excitation/detection wavelengths, nm470/514.5, 532/570 *
Typical measuring time of a single channelno more than 2 sec
Computer interfaceUSB 1.1, RS 232 **
Power consumptionup to 50 W
Dimensions, WõDõH350õ350õ220 mm
Weight15 kg

 

Kits for a PCR amplification and a reverse transcription
  • Formats of PCR amplification kits
  • Base kit
  • Ð format
  • F format (FLASH)™
  • Rt format
  • QRt format
  • Reverse transcription (RT) kit
  • Paraffin
  • Internal control
  • Urogenital infections
  • Hepatitis
  • Other infections
  • Kits for a real-time amplification

    DNA isolation kits
  • Probe-PK
  • Probe-TCh
  • Probe-GS
  • DNA-EXPRESS

    RNA isolation kits
  • "Chomczynski" RNA isolation kit

    Electrophoresis kit
  • ¹ 1
  • ¹ 2

    HLA genotyping kit
  • DNA isolation kit (HLA-genotyping)
  • HLA-DRB1 genotyping kit
  • HLA DQA1 genotyping kit
  • HLA DQB1 genotyping kit
  • DNA detection kit (HLA-genotyping)

    Equipment manufactured by "DNA-Technology" corporation
  • Real-Time PCR Cycler "DT-322"
  • PCR detector "Gene"
  • "Tercyc" PCR Cycler, model 1
  • "Tercyc" PCR Cycler, model 2
  • Five-channel commutator Ê-232
  • Thermostat "Gnom"
  • Thermostat "Thermit"
  • Power supply "Elf 2"
  • Power supply "Elf 4"
  • Power supply "Elf 8"
  • PCR box

    Additional equipment for PCR laboratories
  • Thermostat "Termo 24-15" (Biocom)
  • Thermostat "Ì-206" (Bis-N)
  • Transilluminator ÒÑÐ 15Ì (Vilber Lourmat, France)
  • Transilluminator (Russia)
  • Video system "DNA Analyzer" (Russia)
  • Electrophoresis chamber SE-1 (Helicon)
  • Electrophoresis chamber SE-2 (Helicon)
  • Vortex microcentrifuge "Micro-Spin FV-2400" (Biosan, Latvia)
  • "Pico" centrifuge (Heraeus, Germany)
  • "Ìini Spin" centrifuge (Eppendorf, Germany)
  • PCR box UVC/T-M-AR (Biosan, Latvia)

    Pipettes
  • Semi-automatical pipettes (ThermoLabsystems Color)
  • HTL semi-automatical pipettes (Poland)
  • LABMATE semi-automatical pipettes (Poland)
  • Microman pipette (Gilson)

    Stands
  • "Workplace" tube stand
  • Tube storage stand 30õ1,5 ml
  • Tube stand 90õ0,5 ml
  • Tube storage stand 80õ1,5 ml
  • Pipette stand

    Consumable materials
  • Tubes
  • Pipette tips
  • Other
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    © "DNA-Technology", JSC
    Phone/fax: (495) 727-91-19, 727-94-49, 116-49-02, 117-78-22
    mail@dna-technology.ru
    All equipment and services are certified